Lupin Seed Hydrolysate Promotes G-protein-coupled Receptor, Intracellular Ca²⁺ and Enhanced Glycolytic Metabolism-Mediated Insulin Secretion from BRIN-BD11 Pancreatic Beta Cells

Abstract

Lupin seeds contain bioactive proteins including γ-conglutin that have demonstrated blood glucose-lowering effects, but the cellular mechanisms underlying these effects are not fully understood. This study investigates the mechanisms by which lupin seed hydrolysate promotes insulin secretion from BRIN-BD11 clonal pancreatic beta cells, focusing on G-protein-coupled receptor (GPCR) activation, intracellular calcium signalling, and glycolytic metabolism.

The study demonstrates that lupin seed hydrolysate promotes insulin secretion from BRIN-BD11 pancreatic beta cells through multiple cellular pathways. Activation of G-protein-coupled receptors was identified as a key initiating event, leading to increases in intracellular calcium (Ca²⁺) that are central to the insulin secretion response. Enhanced glycolytic metabolism was also found to contribute to the insulinotropic effect of the lupin hydrolysate. The findings elucidate the molecular mechanisms through which lupin-derived bioactive compounds stimulate insulin release, contributing to the understanding of their potential as functional food ingredients and nutraceuticals for diabetes management.

@article{tapadia2019lupin,
  title         = {Lupin Seed Hydrolysate Promotes G-protein-coupled Receptor,
                  Intracellular Ca2+
                  and Enhanced Glycolytic Metabolism-Mediated Insulin Secretion from BRIN-BD11
                  Pancreatic Beta Cells},
  author        = {Tapadia, Mrunmai and Carlessi, Rodrigo and Johnson, Stuart and Utikar,
                  Ranjeet and Newsholme, Philip},
  year          = 2019,
  journal       = {Molecular and Cellular Endocrinology},
  publisher     = {Elsevier},
  volume        = 480,
  pages         = {83--96},
  doi           = {10.1016/j.mce.2018.10.015}
}